Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck

Pesquisa Agropecuária Brasileira

Marcia Cristina Calixto²  Francisco De Assis Alves Mourão Filho²  Beatriz Madalena Januzzi Mendes¹  Maria Lúcia Carneiro Vieira¹ 

[1] ,Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Dep. de Produção VegetalPiracicaba SP ,Brazil

关键词: genetic improvement;    protoplast fusion;    rootstock;    melhoramento genético;    fusão de protoplastos;    porta-enxerto;   

DOI  :  10.1590/S0100-204X2004000700015

来源: SciELO

【 摘 要 】

This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of 'Hamlin' sweet orange + 'Indian Red' pummelo and 'Hamlin' sweet orange + 'Singapura' pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.

【 授权许可】

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