Polyembryony and identification of Volkamerian lemon zygotic and nucellar seedlings using RAPD

Pesquisa Agropecuária Brasileira

María Andrade-rodríguez¹  Angel Villegas-monter¹  Guillermo Carrillo-castañeda¹  Armando García-velázquez¹ 

[1] ,Colegio de Postgraduados en Ciencias Agrícolas Instituto de Recursos Genéticos y Productividad Montecillo Estado de México ,México

关键词: Citrus volkameriana;    rootstock;    embryo culture;    molecular markers;    polymorphism;    Citrus volkameriana;    porta-enxerto;    cultura de embriões;    marcadores moleculares;    polimorfismo;   

DOI  :  10.1590/S0100-204X2004000600006

来源: SciELO

【 摘 要 】

The objectives of this work were to evaluate the frequency of polyembryony, and to identify zygotic and nucellar seedlings of Citrus volkameriana using RAPD. Twenty-five polyembryonic and eight monoembryonic seeds were cultivated in vitrofor six months. DNA from seedlings was extracted and used in combination with five RAPD primers to identify zygotic or nucellar origin of the seedlings. Environmental conditions of the year affected significantly (P<0.05) the morphological characteristics of fruitsand the number ofembryos per seed. Polyembryonic seeds ranged from 30.9%, 44.8% to 54.4% over three years. Morphological characteristic was not correlated with polyembryony. In vitro culture enable all embryos of each seed to grow, favoring the percentage of seedlings identified as zygotic. In polyembryonic and monoembryonic seeds, 25.9% and 87.5% of the seedlings, respectively, were sexually originated. In polyembryonic seeds, not all zygotic seedlings were produced by small embryos located at the micropyle.

【 授权许可】

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