Sucrose metabolizing enzymes in cell suspension cultures of Bauhinia forficata, Curcuma zedoaria and Phaseolus vulgaris

Pesquisa Agropecuária Brasileira

Marcia Ometto De Mello¹  Antônio Francisco De Campos Amaral¹  Murilo Melo¹ 

[1] ,Escola Superior de Agricultura Luiz de Queiroz Centro de Biotecnologia Agrícola Departamento de Ciências BiológicasPiracicaba SP

关键词: fructokinase;    glycolysis;    tissue culture;    frutofuranosidase;    glicólise;    cultura de tecidos;   

DOI  :  10.1590/S0100-204X2001000900001

来源: SciELO

【 摘 要 】

The objective of this work was to study the activity of sucrose metabolizing enzymes in extracts of cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. Invertase pathway was identified in the three studied species. Sucrose synthase pathway was also responsible for sucrose metabolism in Curcuma zedoaria and Phaseolus vulgaris cells. Activity values higher than 300 nmol min-1 mg-1 of protein were found for acid and neutral invertases, UDPglucose pyrophosphorylase and phosphoglucomutase in the cell extract of the three plant species. Sucrose synthase showed low activity in Bauhinia forficata cells. As sucrose concentration in the culture medium decreased, sucrose synthase activity increased in C. zedoaria and P. vulgaris cells. The glycolytic enzymes activity gradually reduced at the end of the culture period, when carbohydrate was limited.

【 授权许可】

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