Flooding tolerance and cell wall alterations in maize mesocotyl during hypoxia

Pesquisa Agropecuária Brasileira

Patrícia Goulart Vitorino²  José Donizeti Alves²  Paulo César Magalhães¹  Marcelo Murad Magalhães¹  Luíz Carlos Oliveira Lima¹  Luiz Edson Mota De Oliveira² 

[1] ,Universidade Federal de Lavras Departamento de Biologia Lavras MG

关键词: pectic substances;    hemicellulose;    substâncias pécticas;    hemicelulose;   

DOI  :  10.1590/S0100-204X2001000800004

来源: SciELO

【 摘 要 】

This research aimed to characterize the tolerance to flooding and alterations in pectic and hemicellulose fractions from mesocotyl of maize tolerant to flooding when submitted to hypoxia. In order to characterize tolerance seeds from maize cultivars Saracura BRS-4154 and BR 107 tolerant and sensitive to low oxygen levels, respectively, were set to germinate. Plantlet survival was evaluated during five days after having been submitted to hypoxia. After fractionation with ammonium oxalate 0.5% (w/v) and KOH 2M and 4M, Saracura BRS-4154 cell wall was obtained from mesocotyl segments with different damage intensities caused by oxygen deficiency exposure. The cell wall fractions were analyzed by gel filtration and gas chromatography, and also by Infrared Spectrum with Fourrier Transformation (FTIR). The hypoxia period lasting three days or longer caused cell lysis and in advanced stages plant death. The gelic profile from pectic, hemicellulose 2M and 4M fractions from samples with translucid and constriction zone showed the appearance of low molecular weight compounds, similar to glucose. The main neutral sugars in pectic and hemicellulose fractions were arabinose, xilose and mannose. The FTIR spectrum showed a gradual decrease in pectic substances from mesocotyl with normal to translucid and constriction appearance respectively.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License