Revista Brasileira de Ciência do Solo | |
Symbiotic capability of calopo rhizobia from an agrisoil with different crops in Pernambuco | |
Altanys Silva Calheiros1  Mario De Andrade Lira Junior2  Débora Magalhães Soares1  Márcia Do Vale Barreto Figueiredo1  | |
[1] ,Federal Rural University of Pernambuco Agronomy Department Recife PE | |
关键词: Calopogonium mucunoides; biological N fixation; forage legumes; tropical legumes; Calopogonium mucunoides; fixação biológica de N; leguminosas forrageiras; leguminosas tropicais; | |
DOI : 10.1590/S0100-06832013000400005 | |
来源: SciELO | |
【 摘 要 】
Biological nitrogen fixation by rhizobium-legume symbiosis represents one of the most important nitrogen sources for plants and depends strongly on the symbiotic efficiency of the rhizobium strain. This study evaluated the symbiotic capacity of rhizobial isolates from calopo (CALOPOGONIUM MUCUNOIDES) taken from an agrisoil under BRACHIARIA DECUMBENS pasture, sabiá (MIMOSA CAESALPINIIFOLIA) plantations and Atlantic Forest areas of the Dry Forest Zone of Pernambuco. A total of 1,575 isolates were obtained from 398 groups. A single random isolate of each group was authenticated, in randomized blocks with two replications. Each plant was inoculated with 1 mL of a bacterial broth, containing an estimated population of 10(8) rhizobial cells mL-1. Forty-five days after inoculation, the plants were harvested, separated into shoots, roots and nodules, oven-dried to constant mass, and weighed. Next, the symbiotic capability was tested with 1.5 kg of an autoclaved sand:vermiculite (1:1) mixture in polyethylene bags. The treatments consisted of 122 authenticated isolates, selected based on the shoot dry matter, five uninoculated controls (treated with 0, 50, 100, 150, or 200 kg ha-1 N) and a control inoculated with SEMIA 6152 (=BR1602), a strain of BRADYRHIZOBIUM JAPONICUM The test was performed as described above. The shoot dry matter of the plants inoculated with the most effective isolates did not differ from that of plants treated with 150 kg ha-1 N. Shoot dry matter was positively correlated with all other variables. The proportion of effective isolates was highest among isolates from SABIÁ forests. There was great variation in nodule dry weight, as well as in N contents and total N.
【 授权许可】
CC BY
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