Intra-puparial development of the females of Chrysomya albiceps (Wiedemann) (Diptera, Calliphoridae)

Revista Brasileira de Entomologia

José Roberto Pujol-luz¹  Karine Brenda Barros-cordeiro¹ 

[1] ,Universidade de Brasília Instituto de Ciências Biológicas Departamento de ZoologiaBrasília-DF,Brasil

关键词: Forensic entomology;    metamorphosis;    morphology;    Oestroidea;    taxonomy;    Entomologia forense;    metamorfose;    morfologia;    Oestroidea;    taxonomia;   

DOI  :  10.1590/S0085-56262012005000038

来源: SciELO

【 摘 要 】

Intra-puparial development of the females of Chrysomya albiceps (Wiedemann) (Diptera, Calliphoridae). The chronology and morphological changes that take place during intra-puparial development of Chrysomya albiceps is described based on 254 specimens reared in the laboratory. Larvae were obtained from the eggs laid by a single female. The pre-pupae were separated according to the reduction of larval length and the degree of pigmentation and sclerotization of the cuticle. After pupation, 10 individuals were fixed in Carnoy's solution and preserved in 70% ethanol, 10 individuals were fixed every 3 hours up to complete the first 24 hours (n = 80), the remaining individuals were fixed every six hours up to the 90th hour (n = 110) when 54 females emerged. The pupae were immersed in 5% formic acid for 48 hours and maintained in 70% ethanol, and then dissected and analyzed. C. albiceps shows four intra-puparial stages, each of which were described and compared with those described for Musca domestica, Calliphora erythrocephala, Sarcophaga bullata, Cuterebra tenebrosa, Oestrus ovis and Dermatobia hominis. Four developmental stages may be described: (1) the larva-pupa apolysis, after three hours; (2) the criptocephalic pupa, after six hours; (3) the phanerocephalic pupa, after nine hours; (4) the pharate pupa, after nine hours. The pharate adult is completely formed after 81 hours.

【 授权许可】

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