A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivaxandPlasmodium falciparum infection in field-collected anophelines

Memórias do Instituto Oswaldo Cruz

Sara A Bickersmith¹  William Lainhart¹  Marta Moreno¹  Virginia M Chu¹  Joseph M Vinetz¹  Jan E Conn¹ 

关键词: Anopheles;    Plasmodium;    TaqMan;    real-time PCR;   

DOI  :  10.1590/0074-02760150031

来源: SciELO

【 摘 要 】

We describe a simple method for detection of Plasmodium vivaxand Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed withPlasmodium-infected blood meals and in duplicate on field collected An. darlingi. We compared the real-time PCR results of colony-infected and field collected An. darlingi, separately, to a conventional PCR method. We determined that a cytochromeb-PCR method was only 3.33% as sensitive and 93.38% as specific as our real-time PCR assay with field-collected samples. We demonstrate that this assay is sensitive, specific and reproducible.

【 授权许可】

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