Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods

Memórias do Instituto Oswaldo Cruz

Fabiana Martins De Paula¹  Fernanda De Mello Malta¹  Priscilla Duarte Marques¹  Renata Barnabé Sitta¹  João Renato Rebello Pinho¹  Ronaldo César Borges Gryschek¹  Pedro Paulo Chieffi¹ 

关键词: Strongyloides stercoralis;    molecular diagnosis;    conventional PCR;    real-time PCR;   

DOI  :  10.1590/0074-02760140371

来源: SciELO

【 摘 要 】

This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR. The results show that molecular methods can be used as alternative tools for detecting S. stercoralis in human stool samples in tropical areas.

【 授权许可】

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