Iron from haemoglobin and haemin modulates nucleotide hydrolysis inTrichomonas vaginalis

Memórias do Instituto Oswaldo Cruz

Patrícia De Brum Vieira¹  Nícolas Luiz Feijó Silva¹  Luiza Wilges Kist¹  Giovanna Medeiros Tavares De Oliveira¹  Maurício Reis Bogo¹  Geraldo Atillio De Carli¹  Alexandre José Macedo¹  Tiana Tasca¹ 

关键词: Trichomonas vaginalis;    iron;    nucleoside triphosphate diphosphohydrolase;    ecto-5’-nucleotidase;    nucleotide hydrolysis;   

DOI  :  10.1590/0074-02760140320

来源: SciELO

【 摘 要 】

Extracellular ATP may act as a danger signalling molecule, inducing inflammation and immune responses in infection sites. The ectonucleotidases NTPDase and ecto-5’-nucleotidase are enzymes that modulate extracellular nucleotide levels; these enzymes have been previously characterised in Trichomonas vaginalis. Iron plays an important role in the complex trichomonal pathogenesis. Herein, the effects of iron on growth, nucleotide hydrolysis and NTPDase gene expression in T. vaginalisisolates from female and male patients were evaluated. Iron from different sources sustainedT. vaginalis growth. Importantly, iron from haemoglobin (HB) and haemin (HM) enhanced NTPDase activity in isolates from female patients and conversely reduced the enzyme activity in isolates from male patients. Iron treatments could not alter the NTPDase transcript levels in T. vaginalis. Furthermore, our results reveal a distinct ATP, ADP and AMP hydrolysis profile between isolates from female and male patients influenced by iron from HB and HM. Our data indicate the participation of NTPDase and ecto-5’-nucleotidase in the establishment of trichomonas infection through ATP degradation and adenosine production influenced by iron.

【 授权许可】

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