Genetic variation in the Cytbgene of human cerebral Taenia soliumcysticerci recovered from clinically and radiologically heterogeneous patients with neurocysticercosis

Memórias do Instituto Oswaldo Cruz

Héctor Palafox-fonseca¹  Gerardo Zúñiga¹  Raúl José Bobes¹  Tzipe Govezensky¹  Daniel Piñero¹  Laura Texco-martínez¹  Agnès Fleury¹  Jefferson Proaño¹  Graciela Cárdenas¹  Marisela Hernández¹  Edda Sciutto¹  Gladis Fragoso¹ 

关键词: neurocysticercosis;    Taenia solium;    cysticercosis;    genetic variability;   

DOI  :  10.1590/0074-0276130308

来源: SciELO

【 摘 要 】

Neurocysticercosis (NC) is a clinically and radiologically heterogeneous parasitic disease caused by the establishment of larval Taenia solium in the human central nervous system. Host and/or parasite variations may be related to this observed heterogeneity. Genetic differences between pig and human-derived T. solium cysticerci have been reported previously. In this study, 28 cysticerci were surgically removed from 12 human NC patients, the mitochondrial gene that encodes cytochrome b was amplified from the cysticerci and genetic variations that may be related to NC heterogeneity were characterised. Nine different haplotypes (Ht), which were clustered in four haplogroups (Hg), were identified. Hg 3 and 4 exhibited a tendency to associate with age and gender, respectively. However, no significant associations were found between NC heterogeneity and the different T. solium cysticerci Ht or Hg. Parasite variants obtained from patients with similar NC clinical or radiological features were genetically closer than those found in groups of patients with a different NC profile when using the Mantel test. Overall, this study establishes the presence of genetic differences in the Cytb gene of T. solium isolated from human cysticerci and suggests that parasite variation could contribute to NC heterogeneity.

【 授权许可】

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