Schistogram changes after administration of antischistosomal drugs in mice at the early phase of Schistosoma mansoni infection

Memórias do Instituto Oswaldo Cruz

Andréa Cássia Simões Vimieiro¹  Neusa Araújo¹  Naftale Katz¹  John Robert Kusel¹  Paulo Marcos Zech Coelho¹ 

关键词: schistogram;    Schistosoma mansoni;    oxamniquine;    praziquantel;    artesunate;    oogram;   

DOI  :  10.1590/0074-0276130135

来源: SciELO

【 摘 要 】

Mice infected with Schistosoma mansoni were treated with oxamniquine, praziquantel, artesunate at the pre-patent phase, aiming at observing schistogram alterations. Half of the animals were perfused five days post-treatment for counting and classification of immature worms, based on pre-established morphological criteria (schistogram); the remaining animals were evaluated 42 or 100 days after infection and perfusion of the portal-system was performed for collection and counting of adult worms and oogram. It was observed that oxamniquine and artesunate treatment administered at the pre-postural phase causes significant reduction in the number of immature and adult worms. However, there was little reduction with praziquantel when used at the dose of 400 mg/kg for treatments administered 14, 15, 21 or 23 days post-infection. Artesunate was responsible for significant alterations in development of young worms, as well as for a higher number of worms presenting intestinal damages. Immature adult worms were detected in mice treated with artesunate or oxamniquine at the pre-patent phase of infection and recovered by perfusion 100 days after infection. Schistogram proved to be a very useful tool for experimental evaluation of the activity of antischistosomal drugs and a good model to identify the most sensitive stages to drugs.

【 授权许可】

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