Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola

Memórias do Instituto Oswaldo Cruz

Fabio Hiroto Shimabukuro¹  Veruska Maia Da Costa¹  Rodrigo Costa Da Silva¹  Hélio Langoni¹  Aristeu Vieira Da Silva¹  Lídia Raquel De Carvalho¹  Paulo Francisco Domingues¹ 

关键词: microscopic agglutination test;    prozone effect;    leptospirosis;   

DOI  :  10.1590/0074-0276108052013022

来源: SciELO

【 摘 要 】

Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs.

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