Ionic imbalance and lack of effect of adjuvant treatment with methylene blue in the hamster model of leptospirosis

Memórias do Instituto Oswaldo Cruz

Cleiton Silva Santos¹  Everton Cruz De Azevedo¹  Luciane Marieta Soares¹  Magda Oliveira Seixas Carvalho¹  Andreia Carvalho Dos Santos¹  Adenizar Delgado Das Chagas Junior¹  Caroline Luane Rabelo Da Silva¹  Ursula Maira Russo Chagas¹  Mitermayer Galvao Dos Reis¹  Daniel Abensur Athanazio¹ 

关键词: leptospirosis;    methylene blue;    models;    animal;   

DOI  :  10.1590/S0074-0276108042013007

来源: SciELO

【 摘 要 】

Leptospirosis in humans usually involves hypokalaemia and hypomagnesaemia and the putative mechanism underlying such ionic imbalances may be related to nitric oxide (NO) production. We previously demonstrated the correlation between serum levels of NO and the severity of renal disease in patients with severe leptospirosis. Methylene blue inhibits soluble guanylyl cyclase (downstream of the action of any NO synthase isoforms) and was recently reported to have beneficial effects on clinical and experimental sepsis. We investigated the occurrence of serum ionic changes in experimental leptospirosis at various time points (4, 8, 16 and 28 days) in a hamster model. We also determined the effect of methylene blue treatment when administered as an adjuvant therapy, combined with late initiation of standard antibiotic (ampicillin) treatment. Hypokalaemia was not reproduced in this model: all of the groups developed increased levels of serum potassium (K). Furthermore, hypermagnesaemia, rather than magnesium (Mg) depletion, was observed in this hamster model of acute infection. These findings may be associated with an accelerated progression to acute renal failure. Adjuvant treatment with methylene blue had no effect on survival or serum Mg and K levels during acute-phase leptospirosis in hamsters.

【 授权许可】

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