Genetic compatibility between Anopheles lesteri from Korea and Anopheles paraliae from Thailand

Memórias do Instituto Oswaldo Cruz

Kritsana Taai¹  Visut Baimai¹  Atiporn Saeung¹  Sorawat Thongsahuan¹  Gi-sik Min¹  Yasushi Otsuka¹  Mi-hyun Park¹  Masako Fukuda¹  Pradya Somboon¹  Wej Choochote¹ 

关键词: Anopheles lesteri;    Anopheles paraliae;    crossing experiments;    second internal transcribed spacer;    cytochrome c oxidase subunit I;    cytochrome c oxidase subunit II;   

DOI  :  10.1590/S0074-02762013000300009

来源: SciELO

【 摘 要 】

To assess differentiation and relationships between Anopheles lesteri and Anopheles paraliae we established three and five iso-female lines of An. lesteri from Korea and An. paraliae from Thailand, respectively. These isolines were used to investigate the genetic relationships between the two taxa by crossing experiments and by comparing DNA sequences of ribosomal DNA second internal transcribed spacer (ITS2) and mitochondrial DNA cytochrome c oxidase subunit I (COI) and subunit II (COII). Results of reciprocal and F1-hybrid crosses between An. lesteri and An. paraliae indicated that they were compatible genetically producing viable progenies and complete synaptic salivary gland polytene chromosomes without inversion loops in all chromosome arms. The pairwise genetic distances of ITS2, COI and COII between these morphological species were 0.040, 0.007-0.017 and 0.008-0.011, respectively. The specific species status of An. paraliae in Thailand and/or other parts of the continent are discussed.

【 授权许可】

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