Cross-reactive anti-PfCLAG9 antibodies in the sera of asymptomatic parasite carriers of Plasmodium vivax

Memórias do Instituto Oswaldo Cruz

Joana D'arc Neves Costa²  Fernando Berton Zanchi¹  Francisco Lurdevanhe Da Silva Rodrigues²  Eduardo Rezende Honda²  Tony Hiroschi Katsuragawa¹  Dhélio Batista Pereira²  Roger Lafontaine Mesquita Taborda²  Mauro Shugiro Tada²  Ricardo De Godoi Mattos Ferreira¹  Luiz Hildebrando Pereira-da-silva² 

[1] ,Centro de Pesquisa em Medicina TropicalPorto Velho RO ,Brasil

关键词: malaria;    immunity;    Amazon;    cross-reaction;   

DOI  :  10.1590/S0074-02762013000100016

来源: SciELO

【 摘 要 】

The PfCLAG9 has been extensively studied because their immunogenicity. Thereby, the gene product is important for therapeutics interventions and a potential vaccine candidate. Antibodies against synthetic peptides corresponding to selected sequences of the Plasmodium falciparum antigen PfCLAG9 were found in sera of falciparum malaria patients from Rondônia, in the Brazilian Amazon. Much higher antibody titres were found in semi-immune and immune asymptomatic parasite carriers than in subjects suffering clinical infections, corroborating original findings in Papua Guinea. However, sera of Plasmodium vivax patients from the same Amazon area, in particular from asymptomatic vivax parasite carriers, reacted strongly with the same peptides. Bioinformatic analyses revealed regions of similarity between P. falciparum Pfclag9 and the P. vivax ortholog Pvclag7. Indirect fluorescent microscopy analysis showed that antibodies against PfCLAG9 peptides elicited in BALB/c mice react with human red blood cells (RBCs) infected with both P. falciparum and P. vivax parasites. The patterns of reactivity on the surface of the parasitised RBCs are very similar. The present observations support previous findings that PfCLAG9 may be a target of protective immune responses and raises the possibility that the cross reactive antibodies to PvCLAG7 in mixed infections play a role in regulate the fate of Plasmodium mixed infections.

【 授权许可】

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