Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil

Memórias do Instituto Oswaldo Cruz

Vanessa Melandri²  Anthony Érico Guimarães²  Nicholas Komar¹  Maurício L Nogueira¹  Adriano Mondini¹  Ana Fernandez-sesma¹  Jeronimo Alencar²  Irene Bosch¹ 

[1] ,Fiocruz Instituto Oswaldo Cruz Laboratório de DipteraRio de Janeiro RJ ,Brasil

关键词: WNV;    b-ELISA;    PRNT;    Brazil;   

DOI  :  10.1590/S0074-02762012000800020

来源: SciELO

【 摘 要 】

In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.

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