Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil

Memórias do Instituto Oswaldo Cruz

Teresinha Teixeira De Sousa²  Menira Souza²  Fabíola Souza Fiaccadori²  Ana Maria Tavares Borges²  Paulo Sucasas Da Costa¹  Divina Das Dôres De Paula Cardoso² 

[1] ,Instituto de Patologia Tropical e Saúde Pública Laboratório de Virologia Humana

关键词: human bocavirus;    acute gastroenteritis;    molecular characterisation;    Brazil;   

DOI  :  10.1590/S0074-02762012000600015

来源: SciELO

【 摘 要 】

To determine the positivity rate of human bocavirus (HBoV) 1 and 3 among children who presented with acute gastroenteritis symptoms during the period of 1994-2004 in the Central-West Region of Brazil, 762 faecal samples were tested using polymerase chain reaction (PCR) for the detection of HBoV DNA. Primers for a segment of the non-structural viral protein 1 (NS1) gene of HBoV-1 and HBoV-3 were used. Twelve HBoV-positive samples were further characterised via genomic sequencing and phylogenetic analysis. Of the samples tested, 5.8% (n = 44) were positive for HBoV-1 or HBoV-3 and co-infection was observed in 14 (31.8%) of the 44 HBoV-positive samples. Nine of the 14 samples were also positive for Rotavirus A and five were positive for Aichi virus. The genomic sequencing of the NS1 partial sequence of 12 HBoV-samples showed that 11 samples were characterised as HBoV-1 and that one was characterised as HBoV-3. The phylogenetic analysis showed that the HBoV-1 samples had a high sequence homology to others previously identified in China, Sweden and Brazil. This is the first study conducted in the Central-West Region of Brazil to detect HBoV-1 and HBoV-3 in faecal samples from children with acute gastroenteritis. Further studies are required to define the role of HBoVs as aetiological agents of gastroenteritis.

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