Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet

Memórias do Instituto Oswaldo Cruz

Sheisa Cyléia Sargi²  Márcia Machado De Oliveira Dalalio²  Jesuí Vergílio Visentainer²  Rafael Campos Bezerra²  João Ângelo De Lima Perini²  Flávia Braidotti Stevanato¹  Jeane Eliete Laguila Visentainer² 

[1] ,Universidade Estadual de Maringá Departamento de Ciências Básicas da Saúde Laboratório de ImunogenéticaMaringá PR ,Brasil

关键词: macrophages;    Paracoccidioides brasiliensis;    cytokines;    n-3 polyunsaturated fatty acids;   

DOI  :  10.1590/S0074-02762012000300003

来源: SciELO

【 摘 要 】

Omega-3 polyunsaturated fatty acids (n-3 PUFA) can modulate the immune system and their primary effect is on macrophage function. Paracoccidioidomycosis (PCM) is an endemic systemic mycosis in Latin America that is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb). Macrophages are the main defence against this pathogen and have microbicidal activity that is dependent on interferon-Γ and tumour necrosis factor (TNF)-α. These cytokines stimulate the synthesis of nitric oxide (NO) and hydrogen peroxide (H2O2), leading to the death of the fungus. To study the effect of n-3 PUFA on the host immune response during experimental PCM, macrophages that were obtained from animals infected with Pb18 and fed a diet enriched by linseed (LIN) oil were cultured and challenged with the fungus in vitro. The macrophage function was analysed based on the concentrations of TNF-α, NO and H2O2. LIN oil seems to influence the production of TNF-α during the development of disease. A diet enriched with LIN oil influences the microbicidal activity of the macrophages by inducing the production of cytokines and metabolites such as NO and H2O2, predominantly in the chronic phase of infection.

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