Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil

Memórias do Instituto Oswaldo Cruz

Patrícia Martins Parreiras¹  Giovanna Ivo Andrade²  Telma De Figueiredo Do Nascimento²  Maraníbia Cardoso Oelemann²  Harrison Magdinier Gomes²  Andrea Padilha De Alencar²  Ronnie Antunes De Assis²  Pedro Moacyr Pinto Coelho Mota²  Márcia Aparecida Da Silva Pereira²  Francisco Carlos Faria Lobato²  Andrey Pereira Lage²  Philip Noel Suffys² 

[1] ,Instituto René Rachou-FiocruzBelo Horizonte MG ,Brasil

关键词: Mycobacterium bovis;    spoligotyping;    MIRU-VNTR typing;    bovine tuberculosis;    molecular epidemiology;   

DOI  :  10.1590/S0074-02762012000100009

来源: SciELO

【 摘 要 】

We performed spoligotyping and 12-mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTRs) typing to characterise Mycobacterium bovis isolates collected from tissue samples of bovines with lesions suggestive for tuberculosis during slaughter inspection procedures in abattoirs in Brazil. High-quality genotypes were obtained with both procedures for 61 isolates that were obtained from 185 bovine tissue samples and all of these isolates were identified as M. bovis by conventional identification procedures. On the basis of the spoligotyping, 53 isolates were grouped into nine clusters and the remaining eight isolates were unique types, resulting in 17 spoligotypes. The majority of the Brazilian M. bovis isolates displayed spoligotype patterns that have been previously observed in strains isolated from cattle in other countries. MIRU-VNTR typing produced 16 distinct genotypes, with 53 isolates forming eight of the groups, and individual isolates with unique VNTR profiles forming the remaining eight groups. The allelic diversity of each VNTR locus was calculated and only two of the 12-MIRU-VNTR loci presented scores with either a moderate (0.4, MIRU16) or high (0.6, MIRU26) discriminatory index (h). Both typing methods produced similar discriminatory indexes (spoligotyping h = 0.85; MIRU-VNTR h = 0.86) and the combination of the two methods increased the h value to 0.94, resulting in 29 distinct patterns. These results confirm that spoligotyping and VNTR analysis are valuable tools for studying the molecular epidemiology of M. bovis infections in Brazil.

【 授权许可】

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