Participation of N-acetyl-D-glucosamine carbohydrate moieties in the recognition of Schistosoma mansoni sporocysts by haemocytes of Biomphalaria tenagophila

Memórias do Instituto Oswaldo Cruz

Raquel Lopes Martins-souza¹  Cintia Aparecida Jesus Pereira¹  Leonardo Rodrigues²  Emília Souza Araújo¹  Paulo Marcos Zech Coelho¹  Ary Corrêa Jr²  Deborah Negrão-corrêa¹ 

[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de MicrobiologiaBelo Horizonte MG ,Brasil

关键词: Schistosoma mansoni;    Biomphalaria tenagophila;    snail susceptibility to trematode;    N-acetyl-D-glucosamine;    host-parasite recognition;   

DOI  :  10.1590/S0074-02762011000700015

来源: SciELO

【 摘 要 】

Lectin-carbohydrate binding may be involved in the recognition of Schistosoma mansoni sporocysts by haemocytes of Biomphalaria; therefore, we tested if this interaction is associated with snail resistance against Schistosoma infection. In vitro data showed that most of the S. mansoni sporocysts cultured with haemocytes from Biomphalaria glabrata BH, a highly susceptible snail strain, had a low number of cells that adhered to their tegument and a low mortality rate. Moreover, the addition of N-acetyl-D-glucosamine (GlcNAc) did not alter this pattern of adherence and mortality. Using haemocytes and haemolymph of Biomphalaria tenagophila Cabo Frio, we observed a high percentage of sporocysts with adherent cells, but complete encapsulation was not detected. Low concentrations of GlcNAc increased haemocyte binding to the sporocysts and mortality, which returned to basal levels with high concentrations of the carbohydrate. In contrast, haemocytes plus haemolymph from B. tenagophila Taim encapsulated cellular adhesion index of level 3 and destroyed over 30% of the S. mansoni sporocysts in culture. Interestingly, the addition of GlcNAc, but not mannose, to the culture medium resulted in the significant inhibition of cellular adhesion to the parasite tegument and the reduction of parasite mortality, suggesting that GlcNAc carbohydrate moieties are important to the recognition of S. mansoni by B. tenagophila Taim.

【 授权许可】

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