Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction

Memórias do Instituto Oswaldo Cruz

Anahí Souto Vieira²  Grácia Maria Soares Rosinha¹  Carina Elisei De Oliveira¹  Silvio Arruda Vasconcellos¹  Paulo Andre Lima-borges¹  Walfrido Moraes Tomás¹  Guilherme Miranda Mourão¹  Ana Cristina Reis Lacerda¹  Cleber Oliveira Soares¹  Flábio Ribeiro De Araújo¹  Ubiratan Piovezan¹  Carlos Andre Zucco¹  Aiesca Oliveira Pellegrin¹ 

[1] ,Universidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia São Paulo SP ,Brasil

关键词: pampas deer;    Leptospira spp;    MAT;    PCR;    Pantanal;   

DOI  :  10.1590/S0074-02762011000600019

来源: SciELO

【 摘 要 】

This work reports a survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction (PCR). Seventy pampas deer were captured in the dry season and surveyed using PCR, microscopic agglutination test (MAT) (n = 51) and by both techniques (n = 47). PCR detected infections in two pampas deer and MAT detected infections in three. Through sequencing and phylogenetic analyses, the PCR-amplified fragment detected in deer was identified as Leptospira interrogans. Serovars Pomona and Butembo were detected using MAT and the highest titre was 200 for serovar Pomona. Epidemiological aspects of the findings are discussed.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License