Sequence analysis of the 2009 pandemic influenza A H1N1 virus haemagglutinin gene from 2009-2010 Brazilian clinical samples

Memórias do Instituto Oswaldo Cruz

João Leandro De Paula Ferreira¹  Samanta Etel Treiger Borborema¹  Luis Fernando De Macedo Brígido¹  Maria Isabel De Oliveira¹  Terezinha Maria De Paiva¹  Cecília Luiza Simões Dos Santos¹ 

[1] ,Instituto Adolfo Lutz Centro de Virologia São Paulo SP ,Brasil

关键词: influenza;    H1N1;    haemagglutinin;    polymorphism;    Brazil;   

DOI  :  10.1590/S0074-02762011000500014

来源: SciELO

【 摘 要 】

In this paper, we analysed the haemagglutinin (HA) gene identified by polymerase chain reaction from 90 influenza A H1N1 virus strains that circulated in Brazil from April 2009-June 2010. A World Health Organization sequencing protocol allowed us to identify amino acid mutations in the HA protein at positions S220T (71%), D239G/N/S (20%), Y247H (4.5%), E252K (3.3%), M274V (2.2%), Q310H (26.7%) and E391K (12%). A fatal outcome was associated with the D239G mutation (p < 0.0001). Brazilian HA genetic diversity, in comparison to a reference strain from California, highlights the role of influenza virus surveillance for study of viral evolution, in addition to monitoring the spread of the virus worldwide.

【 授权许可】

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