Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing

Memórias do Instituto Oswaldo Cruz

Marcos César Lima De Mendonça²  Ana Maria De Amorim Ferreira¹  Marta Gonçalves Matos Dos Santos¹  Elva Cristina Oviedo¹  Maria Sônia Dal Bello¹  Marilda Mendonça Siqueira¹  Juan Manuel Piñeiro Maceira¹  Maria Genoveva Von Hubinger¹  José Nelson Dos Santos Silva Couceiro¹ 

[1] ,Centro de Ciências da Saúde Instituto de Microbiologia Prof. Paulo de Góes

关键词: parvovirus B19;    nucleotide sequencing;    genotyping;    HMA;    SSCP;   

DOI  :  10.1590/S0074-02762011000400019

来源: SciELO

【 摘 要 】

Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.

【 授权许可】

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