Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal

Memórias do Instituto Oswaldo Cruz

Alex Pauvolid-corrêa²  Maria Alejandra Morales¹  Silvana Levis¹  Luis Tadeu Moraes Figueiredo¹  Dinair Couto-lima²  Zilca Campos¹  Marcia Furlan Nogueira¹  Edson Elias Da Silva¹  Rita Maria Ribeiro Nogueira²  Hermann Gonçalves Schatzmayr² 

[1],Ministério da Saúde Fiocruz Instituto Oswaldo CruzRio de Janeiro RJ ,Brasil

关键词: West Nile virus;    Pantanal;    neutralisation;    equines;    caimans;    BrazilFinancial support;   

DOI  :  10.1590/S0074-02762011000400014

来源: SciELO

【 摘 要 】

Despite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4%) blocking ELISA-seropositive horse serum samples, five (3%) were seropositive for WNV, nine (5.4%) were seropositive for St. Louis encephalitis virus, 18 (10.7%) were seropositive for Ilheus virus, three (1.8%) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of > four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil.

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