Morphological and molecular description of Blastocrithidia cyrtomeni sp. nov. (Kinetoplastea: Trypanosomatidae) associated with Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae) from Colombia

Memórias do Instituto Oswaldo Cruz

Ana Milena Caicedo²  Gerardo Gallego¹  Jaime Eduardo Muñoz¹  Harold Suárez¹  Gerardo Andrés Torres¹  Humberto Carvajal¹  Fanny Caro De Carvajal¹  Andrés Mauricio Posso¹  Dmitriv Maslov¹  James Montoya-lerma² 

[1] ,Universidad del Valle Biology Department Cali Valle ,Colombia

关键词: SL rRNA;    5S rRNA;    SSU rRNA;    trypanosomatids;    Cyrtomenus;    Blastocrithidia cyrtomeni;   

DOI  :  10.1590/S0074-02762011000300008

来源: SciELO

【 摘 要 】

A new trypanosomatid species, Blastocrithidia cyrtomeni, is herein described using morphological and molecular data. It was found parasitising the alimentary tract of the insect host Cyrtomenus bergi, a polyphagous pest. The morphology of B. cyrtomeni was investigated using light and transmission microscopy and molecular phylogeny was inferred from the sequences of spliced leader RNA (SL rRNA) - 5S rRNA gene repeats and the 18S small subunit (SSU) rRNA gene. Epimastigotes of variable size with straphanger cysts adhering to the middle of the flagellum were observed in the intestinal tract, hemolymph and Malpighian tubules. Kinetoplasts were always observed anterior to the nucleus. The ultrastructure of longitudinal sections of epimastigotes showed the flagellum arising laterally from a relatively shallow flagellar pocket near the kinetoplast. SL RNA and 5S rRNA gene repeats were positive in all cases, producing a 0.8-kb band. The amplicons were 797-803 bp long with > 98.5% identity, indicating that they originated from the same organism. According to the sequence analysis of the SL-5S rRNA gene repeats and the 18S SSU rRNA gene, B. cyrtomeni is different from all other known species or isolates of Trypanosomatidae. Both analyses indicate that among known species, it is most closely related to Blastocrithidia triatomae.

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