Genetic and biological characterization of a densovirus isolate that affects dengue virus infection

Memórias do Instituto Oswaldo Cruz

Ana Luiza Pamplona Mosimann²  Juliano Bordignon²  Giovanny Camacho Antevêre Mazzarotto²  Maria Cristina M Motta¹  Federico Hoffmann¹  Claudia Nunes Duarte Dos Santos² 

[1] ,Instituto Carlos Chagas-FiocruzCuritiba PR ,Brasil

关键词: densovirus;    Brevidensovirus;    biological control;    dengue virus;   

DOI  :  10.1590/S0074-02762011000300006

来源: SciELO

【 摘 要 】

Brevidensoviruses have an encapsidated, single-stranded DNA genome that predominantly has a negative polarity. In recent years, they have received particular attention due to their potential role in the biological control of pathogenic arboviruses and to their unnoticed presence in cell cultures as contaminants. In addition, brevidensoviruses may also be useful as viral vectors. This study describes the first genetic and biological characterization of a mosquito densovirus that was isolated in Brazil; moreover, we examined the phylogenetic relationship between this isolate and the other brevidensoviruses. We further demonstrate that this densovirus has the potential to be used to biologically control dengue virus (DENV) infection with in vitro co-infection experiments. The present study provides evidence that this densovirus isolate is a fast-spreading virus that affects cell growth and DENV infection.

【 授权许可】

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