Green propolis phenolic compounds act as vaccine adjuvants, improving humoral and cellular responses in mice inoculated with inactivated vaccines

Memórias do Instituto Oswaldo Cruz

Geferson Fischer²  Niraldo Paulino¹  Maria C Marcucci¹  Bianca S Siedler¹  Lívia S Munhoz¹  Paula F Finger¹  Gilberto D Vargas¹  Sílvia O Hübner¹  Telmo Vidor¹  Paulo M Roehe² 

[1] ,Universidade Federal do Rio Grande do Sul Centro de Ciências Básicas da Saúde

关键词: propolis;    phenolic compounds;    vaccines;    humoral immunity;    cellular immunity;   

DOI  :  10.1590/S0074-02762010000700012

来源: SciELO

【 摘 要 】

Adjuvants play an important role in vaccine formulations by increasing their immunogenicity. In this study, the phenolic compound-rich J fraction (JFR) of a Brazilian green propolis methanolic extract stimulated cellular and humoral immune responses when co-administered with an inactivated vaccine against swine herpesvirus type 1 (SuHV-1). When compared to control vaccines that used aluminium hydroxide as an adjuvant, the use of 10 mg/dose of JFR significantly increased (p < 0.05) neutralizing antibody titres against SuHV-1, as well as the percentage of protected animals following SuHV-1 challenge (p < 0.01). Furthermore, addition of phenolic compounds potentiated the performance of the control vaccine, leading to increased cellular and humoral immune responses and enhanced protection of animals after SuHV-1 challenge (p < 0.05). Prenylated compounds such as Artepillin C that are found in large quantities in JFR are likely to be the substances that are responsible for the adjuvant activity.

【 授权许可】

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