Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement

Memórias do Instituto Oswaldo Cruz

Juliana Magalhães Vital-brazil²  Ilana Teruszkin Balassiano²  Fabiano Sutter De Oliveira¹  Alberto Dias De Souza Costa¹  Leandro Hillen¹  Martha Maria Pereira² 

[1] ,PAHO WHO Centro de Referência Nacional para Leptospirose

关键词: Leptospira spp;    multiplex PCR;    water sample;   

DOI  :  10.1590/S0074-02762010000300020

来源: SciELO

【 摘 要 】

The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies.

【 授权许可】

CC BY   
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