Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells

Memórias do Instituto Oswaldo Cruz

Anderson A Andrade²  Bruno Saf Brasil²  Anna Ctc Pereira¹  Paulo Cp Ferreira²  Erna G Kroon²  Cláudio A Bonjardim² 

[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de MicrobiologiaBelo Horizonte MG ,Brasil

关键词: Vaccinia virus;    virus-host cell interaction;    p21WAF1/Cip1;    MEK/ERK1/2;    signal transduction;   

DOI  :  10.1590/S0074-02762010000300005

来源: SciELO

【 摘 要 】

In this paper, we provide evidence that both the mRNA and protein levels of the cyclin-dependent kinase (CDK) inhibitor p21WAF1/CDK-interacting protein 1 (Cip1) increase upon infection of A431 cells with Vaccinia virus (VACV). In addition, the VACV growth factor (VGF) seems to be required for the gene expression because infection carried out with the mutant virus VACV-VGF- revealed that this strain was unable to stimulate its transcription. Our findings are also consistent with the notion that the VGF-mediated change in p21WAF1/Cip1 expression is dependent on tyrosine kinase pathway(s) and is partially dependent on mitogen-activated protein kinase/extracellular-signal regulated kinase 1/2. We believe that these pathways are biologically significant because VACV replication and dissemination was drastically affected when the infection was carried out in the presence of the relevant pharmacological inhibitors.

【 授权许可】

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