Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells

Memórias do Instituto Oswaldo Cruz

Fernanda F Rocha¹  Ricardo N Araujo¹  Luciana M Silva²  Nelder F Gontijo¹  Marcos H Pereira¹ 

[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Departamento de ParasitologiaBelo Horizonte MG ,Brasil

关键词: Rhodnius prolixus;    salivary gland;    primary culture;   

DOI  :  10.1590/S0074-02762010000200004

来源: SciELO

【 摘 要 】

In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role.

【 授权许可】

CC BY   
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