Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre

Memórias do Instituto Oswaldo Cruz

Aline Fagundes¹  Armando Schubach¹  Cíntia Cristiane De Paula¹  Alessandra Bogio¹  Liliane De Fátima Antonio¹  Patrícia Botelho Schiavoni¹  Vivian De Souza Monteiro¹  Maria De Fátima Madeira¹  Leonardo Pereira Quintella²  Claudia Maria Valete-rosalino¹  Erica De Camargo Ferreira E Vasconcellos¹  Rilza Beatriz Gayoso De Azeredo-coutinho¹  Rachel S Pacheco¹  Mauro Ca Marzochi¹  Keyla Bf Marzochi¹ 

[1] ,Instituto de Pesquisa Clínica Evandro Chagas Serviço de Anatomia Patológica

关键词: Leishmania;    polymerase chain reaction;    diagnosis;   

DOI  :  10.1590/S0074-02762010000100018

来源: SciELO

【 摘 要 】

The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.

【 授权许可】

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