Do Archaea and bacteria co-infection have a role in the pathogenesis of chronic chagasic cardiopathy?

Memórias do Instituto Oswaldo Cruz

Maria De Lourdes Higuchi¹  Joyce Kawakami¹  Renata Ikegami¹  Maysa Beatriz Mandetta Clementino¹  Flavio M Kawamoto¹  Marcia M Reis¹  Edimar Bocchi¹ 

[1] ,Universidade de São Paulo Faculdade de Medicina Instituto do CoraçãoSão Paulo SP ,Brasil

关键词: Chlamydia pneumoniae;    Mycoplasma pneumoniae;    Archaea;    Chagas disease;    complement C5b-9;   

DOI  :  10.1590/S0074-02762009000900026

来源: SciELO

【 摘 要 】

Chronic cardiopathy (CC) in Chagas disease is a fibrotic myocarditis with C5b-9 complement deposition. Mycoplasma and Chlamydia may interfere with the complement response. Proteolytic enzymes and archaeal genes that have been described in Trypanosoma cruzi may increase its virulence. Here we tested the hypothesis that different ratios of Mycoplasma, Chlamydia and archaeal organisms, which are frequent symbionts, may be associated with chagasic clinical forms. MATERIALS AND METHODS: eight indeterminate form (IF) and 20 CC chagasic endomyocardial biopsies were submitted to in situ hybridization, electron and immunoelectron microscopy and PCR techniques for detection of Mycoplasma pneumoniae (MP), Chlamydia pneumoniae(CP), C5b-9 and archaeal-like bodies. RESULTS: MP and CP-DNA were always present at lower levels in CC than in IF (p < 0.001) and were correlated with each other only in CC. Electron microscopy revealed Mycoplasma, Chlamydia and two types of archaeal-like bodies. One had electron dense lipid content (EDL) and was mainly present in IF. The other had electron lucent content (ELC) and was mainly present in CC. In this group, ELC correlated negatively with the other microbes and EDL and positively with C5b-9. The CC group was positive for Archaea and T. cruzi DNA. In conclusion, different amounts of Mycoplasma, Chlamydia and archaeal organisms may be implicated in complement activation and may have a role in Chagas disease outcome.

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