Molecular characterization of enterococci harboring genotype and phenotype incongruence related to glycopeptide resistance isolated in Brazilian hospitals

Memórias do Instituto Oswaldo Cruz

Priscila Moraes Henrique²  Izabel Cristina Vanzato Palazzo²  Rosemeire Cobo Zanella¹  Ana Lucia Da Costa Darini² 

[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas Departamento de Análises Clínicas, Toxicológicas e BromatológicasRibeirão Preto SP ,Brasil

关键词: vancomycin resistant enterococcci (VRE);    vanA genotype;    VanB phenotype;   

DOI  :  10.1590/S0074-02762008000300014

来源: SciELO

【 摘 要 】

Three Enterococcus faecalis and one Enterococcus faecium strains were characterized by plasmid profile, pulsed-field gel electrophoresis (PFGE) and determination of antimicrobial minimal inhibitory concentrations. VanA elements were characterized by Long PCR, overlapping PCR and DNA sequencing. Enterococcal strains showed resistance to vancomycin and harbored the vanA gene, and three these were teicoplanin susceptible while one showed intermediate resistance to teicoplanin. Two E. faecalis strains showed indistinguishable PFGE profile while the third was unrelated. E. faecalis strains showed a deletion in the right terminal region of the Tn1546-like element. The E. faecium strain showed an insertion element in the vanXY intergenic region. Mutations in VanA elements were not found. Rearrangements in the VanA element could be responsible for incongruities in genotype and phenotype in these strains.

【 授权许可】

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