Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference

Memórias do Instituto Oswaldo Cruz

Liliana Torcoroma García²  Ney Ribeiro Leite²  Juan D Alfonzo¹  Otavio Henrique Thiemann¹ 

[1],Universidade de São Paulo Instituto de Física de São Carlos São Carlos SP ,Brasil

关键词: tryptophanyl tRNA synthetase;    RNA interference;    kinetoplastid;    Trypanosoma bruce;   

DOI  :  10.1590/S0074-02762007005000090

来源: SciELO

【 摘 要 】

The kinetoplast genetic code deviates from the universal code in that 90% of mitochondrial tryptophans are specified by UGA instead of UGG codons. A single nucleus-encoded tRNA Trp(CCA) is used by both nuclear and mitochondria genes, since all kinetoplast tRNAs are imported into the mitochondria from the cytoplasm. To allow decoding of the mitochondrial UGA codons as tryptophan, the tRNA Trp(CCA) anticodon is changed to UCA by an editing event. Two tryptophanyl tRNA synthetases (TrpRSs) have been identified in Trypanosoma brucei: TbTrpRS1 and TbTrpRS2 which localize to the cytoplasm and mitochondria respectively. We used inducible RNA interference (RNAi) to assess the role of TbTrpRSs. Our data validates previous observations of TrpRS as potential drug design targets and investigates the RNAi effect on the mitochondria of the parasite.

【 授权许可】

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