Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags

Memórias do Instituto Oswaldo Cruz

Luciana Girotto Gentil¹  Fernanda Lasakosvitsch¹  José Franco Da Silveira¹  Márcia Regina Machado Dos Santos¹  Clara Lúcia Barbiéri¹ 

[1] ,Universidade Federal de São Paulo Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e ParasitologiaSão Paulo SP ,Brasil

关键词: Leishmania (Leishmania) amazonensis;    amastigotes;    cDNA library;   

DOI  :  10.1590/S0074-02762007005000065

来源: SciELO

【 摘 要 】

The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome localization of some L. (L.) amazonensis ESTs was also determined in L. (L.) amazonensis and L. (L.) major. The characterization of these ESTs is suitable for the genome physical mapping, as well as for the identification of genes encoding cysteine proteinases implicated with protective immune responses in leishmaniasis.

【 授权许可】

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