Identification of excreted iron superoxide dismutase for the diagnosis of Phtytomonas

Memórias do Instituto Oswaldo Cruz

Clotilde Marín¹  Isabel Rodríguez-gonzález¹  Manuel Sánchez- Moreno¹ 

[1] ,Universidad de Granada Facultad de Ciencias Departamento de ParasitologíaGranada,España

关键词: superoxide dismutase;    immunogenicity;    molecular tool;    Phytomonas spp;   

DOI  :  10.1590/S0074-02762006000600012

来源: SciELO

【 摘 要 】

An excreted iron superoxide dismutase (FeSODe) of pI 3.6 with a molecular weight of 28-30 kDa was detected in the in vitro culture of Phytomonas isolated from Euphorbia characias (SODeCHA) and from Lycopersicon esculentum (SODeTOM), in Grace's medium without serum. These FeSODe excreted into the medium had immunogenic capacity: the positivity of the anti-SODeCHA serum persisted to a dilution of 1/30,000, and for the anti-SODeTOM to 1/10,000 by Western blot. In addition, cross reaction was detected between the anti-SODe serum of Phytomonas isolated from E. characias against SODeTOM, and the anti-SODe serum from L. esculentum with SODeCHA. This characteristic offers the possibility of its use to diagnose plant trypanosomatids. The validation of the test was confirmed by experimental inoculation of tomato fruits with Phytomonas isolated from L. esculentum. At 7, 10, 15, and 21 days post infection, it was possible to detect the presence of the parasites with the anti-SODe serum of Phytomonas isolated from L. esculentum at a dilution of 1/250. These serological results were confirmed by visualization of the parasites by optical microscopy. The data of this study confirm that the SOD is sufficient to identify a trypanosomatid isolated from plants as belonging to the genus Phytomonas.

【 授权许可】

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