Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale

Memórias do Instituto Oswaldo Cruz

Flábio R Araújo²  Valeska Sp Melo¹  Carlos An Ramos²  Claudio R Madruga²  Cleber O Soares²  Raul H Kessler²  Nalvo F Almeida¹  Graziela S Araújo¹  Leucio C Alves¹  Roberto Aa Torres Júnior²  Stênio P Fragoso¹  Paulo Rc Arauco¹  Gisele Bacanelli²  Maristeli B Oliveira²  Lenita R Santos¹ 

[1] ,Embrapa Gado de CorteCampo Grande MS ,Brasil

关键词: Anaplasma marginale;    indirect enzyme-linked immunosorbent assays;    major surface proteins;    Brazil;   

DOI  :  10.1590/S0074-02762005000700016

来源: SciELO

【 摘 要 】

Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. The high sensitivities (99% for both tests) and specificities (100% for both tests) were confirmed with sera from cattle positive or negative for A. marginale antibodies, respectively, by immunofluorescent antibody test. By the analysis of 583 sera from cattle of three regions of the state of Pernambuco, Brazil, the agreement between both tests was high, with a kappa index of 0.89. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle.

【 授权许可】

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