Isolation of a fragment homologous to the rp49 constitutive gene of Drosophila in the Neotropical malaria vector Anopheles aquasalis (Diptera: Culicidae)

Memórias do Instituto Oswaldo Cruz

C Gentile²  Jbp Lima¹  Aa Peixoto² 

[1] ,Fiocruz Instituto Oswaldo Cruz Departamento de Bioquímica e Biologia MolecularRio de Janeiro RJ ,Brasil

关键词: Anopheles aquasalis;    Aedes aegypti;    Culex quinquefasciatus;    Drosophila constitutive gene;    rp49;    rpL32;    mosquitoes;   

DOI  :  10.1590/S0074-02762005000600008

来源: SciELO

【 摘 要 】

The constitutive ribosomal gene rp49 is frequently used as an endogenous control in Drosophila gene expression experiments. Using the degenerate primer PCR technique we have cloned a fragment homologous to this gene in Anopheles aquasalis Curry, a Neotropical vector of malaria. In addition, based on this first sequence, a new primer was designed, which allowed the isolation of fragments of rp49 in two other species, Aedes aegypti (Linnaeus) and Culex quinquefasciatus Say, suggesting that it could be used to clone fragments of this gene in a number of other mosquito species. Primers were also designed to specifically amplify rp49 cDNA fragments in An. aquasalis and Ae. aegypti, showing that rp49 could be used as a good constitutive control in gene expression studies of these and other vectorially important mosquito species.

【 授权许可】

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