Phenotypic, functional, and quantitative characterization of canine peripheral blood monocyte-derived macrophages

Memórias do Instituto Oswaldo Cruz

R Bueno²  Mn Mello¹  Cas Menezes¹  Wo Dutra¹  Rl Santos² 

[1] ,Universidade Federal de Minas Gerais ICB Escola de VeterináriaBelo Horizonte MG ,Brasil

关键词: monocyte-derived macrophage;    canine;    flow-cytometry;   

DOI  :  10.1590/S0074-02762005000500012

来源: SciELO

【 摘 要 】

The yield as well as phenotypic and functional parameters of canine peripheral blood monocyte-derived macrophages were analyzed. The cells that remained adherent to Teflon after 10 days of culture had high phagocytic activity when inoculated with Leishmania chagasi. Flow cytometric analysis demonstrated that more than 80% of cultured cells were positive for the monocyte/macrophage marker CD14.

【 授权许可】

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