Analysis of expressed sequence tags from Trypanosoma cruzi amastigotes

Memórias do Instituto Oswaldo Cruz

Gustavo C Cerqueira¹  Wanderson D Darocha¹  Priscila C Campos¹  Cláudia S Zouain¹  Santuza Mr Teixeira¹ 

[1] ,Universidade Federal de Minas Gerais ICB Departamento de Bioquímica e ImunologiaBelo Horizonte MG ,Brasil

关键词: Trypanosoma cruzi;    gene expression;    amastigotes;    antigens;   

DOI  :  10.1590/S0074-02762005000400008

来源: SciELO

【 摘 要 】

A total of 880 expressed sequence tags (EST) originated from clones randomly selected from a Trypanosoma cruzi amastigote cDNA library have been analyzed. Of these, 40% (355 ESTs) have been identified by similarity to sequences in public databases and classified according to functional categorization of their putative products. About 11% of the mRNAs expressed in amastigotes are related to the translational machinery, and a large number of them (9% of the total number of clones in the library) encode ribosomal proteins. A comparative analysis with a previous study, where clones from the same library were selected using sera from patients with Chagas disease, revealed that ribosomal proteins also represent the largest class of antigen coding genes expressed in amastigotes (54% of all immunoselected clones). However, although more than thirty classes of ribosomal proteins were identified by EST analysis, the results of the immunoscreening indicated that only a particular subset of them contains major antigenic determinants recognized by antibodies from Chagas disease patients.

【 授权许可】

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