In vitro measurement of enzymatic markers as a tool to detect mouse cardiomyocytes injury

Memórias do Instituto Oswaldo Cruz

Elen Mello De Souza²  Andrea Henriques-pons²  Christian Bailly¹  Amelie Lansiaux¹  Tânia Cremonine Araújo-jorge²  Maria De Nazaré Correia Soeiro² 

[1] ,Instituto Oswaldo Cruz-Fiocruz Departamento de Ultra-estrutura e Biologia Celular Laboratório de Biologia CelularRio de Janeiro RJ ,Brasil

关键词: creatine kinase;    in vitro assays;    cardiac damage;   

DOI  :  10.1590/S0074-02762004000700006

来源: SciELO

【 摘 要 】

Primary cultures of cardiomyocytes represent a useful model for analyzing cardiac cell biology as well as pathogenesis of several cardiovascular disorders. Our aim was to standardize protocols for determining the damage of cardiac cells cultured in vitro by measuring the creatine kinase and its cardiac isotype and lactate dehydrogenase activities in the supernatants of mice cardiomyocytes submitted to different protocols of cell lysis. Our data showed that due to its higher specificity, the cardiac isotype creatine kinase was the most sensitive as compared to the others studied enzymatic markers, and can be used to monitor and evaluate cardiac damage in in vitro assays.

【 授权许可】

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