The use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of Lymnaea columella, L. viatrix, and L. diaphana (Mollusca: Lymnaeidae)

Memórias do Instituto Oswaldo Cruz

Omar S Carvalho²  Paula Cm Cardoso²  Pollanah M Lira²  Alejandra Rumi¹  Andrea Roche¹  Elizabeth Berne¹  Gertrud Müller¹  Roberta L Caldeira² 

[1] ,Centro de Pesquisas René Rachou-Fiocruz Laboratório de Helmintoses Intestinais Belo Horizonte MG ,Brasil

关键词: Lymnaea columella;    Lymnaea viatrix;    Lymnaea diaphana;    polymerase chain reactionandrestriction fragment length polymorphism;    first and second internal transcribed spacers of the RNA ribosomal gene;    subunit 16S of mitochondrial RNA ribosomal gene;   

DOI  :  10.1590/S0074-02762004000500008

来源: SciELO

【 摘 要 】

The specific identification of Lymnaeid snails is based on a comparison of morphological characters of the shell, radula, renal and reproductive organs. However, the identification is complicated by dissection process, intra and interspecific similarity and variability of morphological characters. In the present study, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) techniques targeted to the first and second internal transcribed spacers (ITS1 and ITS2) rDNA and to the mitochondrial 16S ribosomal gene (16S rDNAmt) were used to differentiate the species Lymnaea columella, L. viatrix, and L. diaphana from some localities of Brazil, Argentina, and Uruguay as well as to verify whether the molecular results corroborates the classical morphological method.PCR-RFLP analysis of the ITS1, ITS2, and 16S using 12 restriction enzymes revealed characteristic patterns for L. columella and L. diaphana which were concordant with the classical morphology. On the other hand, for L. viatrix populations a number of 1 to 6 profiles were generated while morphology provided the species pattern results.

【 授权许可】

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