Dominant character of the molecular marker of a Biomphalaria tenagophila strain (Mollusca: Planorbidae) resistant to Schistosoma mansoni

Memórias do Instituto Oswaldo Cruz

Florence Mara Rosa²  Roberta Lima Caldeira¹  Omar Dos Santos Carvalho¹  Ana Lúcia Brunialti Godard¹  Paulo Marcos Zech Coelho¹ 

[1] ,UFMG Instituto de Ciências Biológicas Departamento de ParasitologiaBelo Horizonte MG ,Brasil

关键词: Biomphalaria tenagophila;    polymerase chain reaction and restriction fragment length polymorphism;    dominant character;    Taim;    Rio Grande do Sul - Brazil;   

DOI  :  10.1590/S0074-02762004000100015

来源: SciELO

【 摘 要 】

Biomphalaria tenagophila population from Taim (state of Rio Grande do Sul, Brazil) is totally resistant toSchistosoma mansoni, and presents a molecular marker of 350 bp by polymerase chain reaction and restriction fragment length polymorphism of the entire rDNA internal transcriber spacer. The scope of this work was to determine the heritage pattern of this marker. A series of cross-breedings between B. tenagophila from Taim (resistant) and B. tenagophila from Joinville, state of Santa Catarina (susceptible) was carried out, and their descendants F1 and F2 were submitted to this technique. It was possible to demonstrate that the specific fragment from Taim is endowed with dominant character, since the obtained segregation was typically mendelian.

【 授权许可】

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