Whole blood assay to access T cell-immune responses to Mycobacterium tuberculosis antigens in healthy Brazilian individuals

Memórias do Instituto Oswaldo Cruz

Paulo Rz Antas²  Fernando Ll Cardoso²  Eliane B Oliveira²  Patrícia Kc Gomes²  Kátia S Cunha¹  Euzenir N Sarno²  Elizabeth P Sampaio² 

[1],Instituto Oswaldo Cruz-Fiocruz Laboratório de Hanseníase Rio de Janeiro RJ ,Brasil

关键词: tuberculosis (TB);    T cell-responses;    whole blood;    PPD;    latent TB infection;    interferon gamma;   

DOI  :  10.1590/S0074-02762004000100009

来源: SciELO

【 摘 要 】

The production of interferon gamma (IFNgamma) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens in terms of IFNg production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r² = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.

【 授权许可】

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