Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay

Memórias do Instituto Oswaldo Cruz

Eleuza Rodrigues Machado²  Marlene Tiduko Ueta²  Maria Do Rosário De Fátima Gonçalves-pires¹  João Batista Alves De Oliveira²  Lúcia Helena Faccioli¹  Julia Maria Costa-cruz¹ 

[1] ,Universidade Estadual de Campinas Instituto de Biologia Departamento de Parasitologia SP ,Brazil

关键词: Strongyloides venezuelensis;    strongyloidiasis;    diagnosis;    enzyme-linked immunosorbent assay;   

DOI  :  10.1590/S0074-02762003000600024

来源: SciELO

【 摘 要 】

The present study was conducted to detected IgG antibodies using Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by the enzyme-linked immunosorbent assay (ELISA). Sera from 90 subjects were analyzed (30 with strongyloidiasis, 30 with other parasites and 30 healthy individuals). Results were expressed in antibody titers, which were considered as positive when titer was > 80. Sensibility and specificity of the assay were 100% and 96.7%, respectively. It can be concluded that the heterologous alkaline extract could be employed in ELISA as a diagnostic aid in human strongyloidiasis, due to its advantages as easiness of obtaining, practicability in preparing, and high indexes of sensitivity and specificity.

【 授权许可】

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