The ue of polysiloxane/polyvinyl alcohol beads as solid phase in IgG anti-Toxocara canis detection using a recombinant antigen

Memórias do Instituto Oswaldo Cruz

Raquel De Andrade Lima Coêlho²  Hiroshi Yamasaki¹  Emília Perez¹  Luiz Bezerra De Carvalho Jr² 

[1] ,Universidade Federal de Pernambuco Departamento de Bioquímica Laboratório de Imunopatologia Keizo AsamiRecife PE ,Brasil

关键词: polysiloxane;    polyvinyl alcohol;    recombinant antigen;    Toxocara canis;   

DOI  :  10.1590/S0074-02762003000300018

来源: SciELO

【 摘 要 】

Immunodetection of human IgG anti-Toxocara canis was developed based on ELISA and on the use of polysiloxane/polyvinyl alcohol (POS/PVA) beads. A recombinant antigen was covalently immobilized, via glutaraldehyde, onto this hybrid inorganic-organic composite, which was prepared by the sol-gel technique. Using only 31.2 ng antigen per bead, a peroxidase conjugate dilution of 1:10,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. This procedure is comparable to that which utilizes the adsorption of the antigen to conventional PVC plates. However, the difference between positive and negative sera mean absorbances was larger for this new glass based assay. In addition to the performance of the POS/PVA bead as a matrix for immunodetection, its easy synthesis and low cost are additional advantages for commercial application.

【 授权许可】

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