Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes

Memórias do Instituto Oswaldo Cruz

Omar A Saldarriaga²  Jaime I Velásquez¹  Jorge E Ossa²  Maria T Rugeles² 

[1] ,Universidad de Antioquia Facultad de Medicina Grupo de Inmunovirologia-Biogenesis

关键词: macrophages;    bovine;    trypanosomes;   

DOI  :  10.1590/S0074-02762003000200017

来源: SciELO

【 摘 要 】

We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License