Genetic markers between Biomphalaria glabrata snails susceptible and resistant to Schistosoma mansoni infection

Memórias do Instituto Oswaldo Cruz

Rgm Spada²  D Da Silva²  A-z Abdel-hamid¹  Ss Sobral-hamaguchi²  Nrb Zuim¹  Em Zanotti-magalhães¹  La Magalhães¹  Jt Ribeiro-paes² 

[1] ,Unesp Departamento de Ciências Biológicas Assis SP ,Brasil

关键词: Schistosoma;    Biomphalaria;    genetic variability;    polymorphism;    random amplified polymorphic DNA;    polymerase chain reaction;   

DOI  :  10.1590/S0074-02762002000900012

来源: SciELO

【 摘 要 】

The analysis of the genetic variability related to susceptibility to Schistosoma mansoni infection in the vector of the genus Biomphalaria is important in terms of a better understanding of the epidemiology of schistosomiasis itself, the possible pathological implications of this interaction in vertebrate hosts, and the formulation of new strategies and approaches for disease control. In the present study, the genetic variability of B. glabrata strains found to be resistant or susceptible to S. mansoni infection was investigated using DNA amplification by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The amplification products were analyzed on 8% polyacrylamide gel and stained with silver. We selected 10 primers, since they have previously been useful to detect polymorphism among B. glabrata and/or B. tenagophila. The results showed polymorphisms with 5 primers. Polymorphic bands observed only in the susceptible strain. The RAPD-PCR methodology represents an adequate approach for the analysis of genetic polymorphisms. The understanding of the genetic polymorphisms associated to resistance may contribute to the future identification of genomic sequences related to the resistance/susceptibility of Biomphalaria to the larval forms of S. mansoni and to the development of new strategies for the control of schistosomiasis.

【 授权许可】

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