Identification of snails within the Bulinus africanus group from East Africa by multiplex SNaPshotTManalysis of single nucleotide polymorphisms within the cytochrome oxidase subunit I

Memórias do Instituto Oswaldo Cruz

Jr Stothard²  J Llewellyn-hughes²  Ce Griffin²  Sj Hubbard¹  Tk Kristensen¹  D Rollinson² 

[1] ,The Natural History Museum Department of Zoology Wolfson Wellcome Biomedical LaboratoriesLondon

关键词: Bulinus;    cytochrome oxidase;    single nucleotide polymorphism;    schistosomiasis;    Schistosoma haematobium;   

DOI  :  10.1590/S0074-02762002000900008

来源: SciELO

【 摘 要 】

Identification of populations of Bulinus nasutus and B. globosus from East Africa is unreliable using characters of the shell. In this paper, a molecular method of identification is presented for each species based on DNA sequence variation within the mitochondrial cytochrome oxidase subunit I (COI) as detected by a novel multiplexed SNaPshotTM assay. In total, snails from 7 localities from coastal Kenya were typed using this assay and variation within shell morphology was compared to reference material from Zanzibar. Four locations were found to contain B. nasutus and 2 locations were found to contain B. globosus. A mixed population containing both B. nasutus and B. globosus was found at Kinango. Morphometric variation between samples was considerable and UPGMA cluster analysis failed to differentiate species. The multiplex SNaPshotTM assay is an important development for more precise methods of identification of B. africanus group snails. The assay could be further broadened for identification of other snail intermediate host species.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License