Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain

Memórias do Instituto Oswaldo Cruz

Ana Le-senne¹  Susana Muelas-serrano¹  Carlos Fernández-portillo¹  José Antonio Escario¹  Alicia Gómez-barrio¹ 

[1] ,Universidad Complutense Facultad de Farmacia Departamento de ParasitologíaMadrid,España

关键词: Trypanosoma cruzi;    clone CL B5;    biological characterization;   

DOI  :  10.1590/S0074-02762002000800006

来源: SciELO

【 摘 要 】

Clone CL B5 of Trypanosoma cruzi is a beta-galactosidase expressing organism that was genetically transfected to be used for in vitro pharmacological screening. Biological parameters were determined, evaluating growth kinetics of epimastigotes, metacyclogenesis, infectivity to mammalian cell lines, parasitemia kinetics in mice and sensibility to nifurtimox and benznidazole. Differences in relation to other strains and CL parental strain were found, the most important being the incapability to produce death to mice in spite of the high inoculum used. However, it possesses the required features to be used for in vitro drug screening. Data obtained demonstrate that heterogeneity of T. cruzi appears even among clones of the same strain, and that these differences found do not prevent the use of clone CL B5 for the purpose that was engineered.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License